Well I am not a great scientist to tell the truth. I was a pretty bad student in school, any teacher I had can vouch for that. I think it was from my experimenting and chemistry with females that lead to the science failure in school.
When I was much younger I had a laboratory in the basement and was known for creating some pretty explosive experiments after a few miscalculations my lab was permanently moved to the garage.
Now that I am into growing plants I find myself using chemistry much more than I had expected and some of the things my much smarter friends have been telling me sound truly amazing. That is why I am taking up this experiment. To most people Tetriploids don't mean a whole lot it manly means that a plant has double the chromosomes. Which usually means a mutant or deformity. But for the plant collectors this is usually a good thing the more chromosomes usually the larger the flowers. Now as I understand it most plants have two chromosomes one from the mother and one from the father. Some plants either through breeding or mutations may have three chromosomes hens tetriploid. Now as my friend tells me if the number of chromosomes are a even number the plant can reproduce sexually and create seeds. But if the plant had a odd number of chromosomes the plant is usually sterile and will not produce any seeds. This is were the tetriploid conversion is highly needed. For instance I plan to work on cannas and one canna I know is a tetriploid is Bengal tiger. What I would do is take a small rhizome and soak it in this solution for one night and rinse off the next day. Once it recovers from this chemical treatment and hopefully survives. It will then if all goes well have doubled the chromosomes so taking 3 chromosomes and changing it to 6 I now would have a plant capable of reproducing sexually. It would also work great on seedlings and doubling the chromosomes would mean thicker leaves much larger flowers and in some cases odd coloration's.
This sounds like something some lab would be working on but truthfully I believe almost if not all research and applications have been by in home scientist. Most people would think this is unnatural but it does happen naturally through tons of breeding much like some fruit trees no longer have seeds due to breeding for certain traits. I have seen the most impressive use of this in day lilies were huge flowering forms and odd ruffling and colors are seen in the flowers. It has taken day lilies to a whole new ground breaking level of breeding. I hope to now use this technic on other groups of plants seldom worked with.
I had heard of chemical conversions before years ago and it did peak my interest. The problem was the chemicals being used to create mutations could also create mutations in humans leading to cancer. This really made me stay away from this work but it seems now much safer chemicals are being used with even better results than the more dangerous chemicals. So here I am now with my test tubes and rubber gloves on ready to manipulate tons of my new hybrid seedlings. I will post more on my out comes and if interested more information on the chemicals and process used in doubling the chromosomes. Hopefully by spring I will have some interesting results. Anyone else with their mad scientist labs I would love to hear. THANKS
Tetriploid conversion AKA mad scientest experiment
Oh my gosh Brian, that's stunning. Your experiments with plants is fascinating. Thanks for sharing what you're doing with us, and please keep us up with your results. Does that one have a name?
Sounds aweseom! Can't wait to see the results.
This is canna Florence Vaughn it is a well known tetriploid and with many breeders you hit this wall were you breed something extremely beautiful that you would love to expand on only to find out that its sterile. So if all works well conversion with this plant would make it possible to get seeds and breed further with its genetics.
Conversion and breeding would take one season it would take another season to grow out the seedlings. I would love to get this flower on a dark leaf canna and it is one of my goals. If you have seen my cannas before I have delt manly with foliage I am now starting to work more on the flowers on top of the more unusual foliage forms.
Beautiful!!!!!!!!! What a lovely thread. You don't know how glad I am to se that there is somebody else beside s me thignign out side of the box and creating experiments that most think should not be possible, but how we ever gonna know if some of us don't try.
The hard part with the conversions is gettign them to stick and not convert back and only time and probably checking the size of the pollen will.
Way to go!!! Aren't mutants fun to work with?????? LOL Just don't blow up your experiments. : )
Brian..sounds great... I know from your results in growing and breeding in a more standard manner... you'll havesome interesting results here... get us up to speed on these methods... and perhaps I'll join you in the work... Gordon
Gordon I am using a treflan and DMSO chemical mix. From what I gather Treflan with the active ingredient trifluralin is a weed preventive but causes chromosome doubling once a seed has sprouted the dose is very very small. Then you add DMSO it is probably the most dangerous chemical in the mix and it mainly will help anything absorb. So if you use it use gloves and protection with it. It use to be used in athritis cream but I had to special order it off the internet. All the work with the plants should be done in the dim to dark area and once the plants are cleaned and replanted it should be kept fairly dim they will be sensitive to light. Here is a website on conversion I am using a paper given to be by a friend but have found this site very useful. But truthfully I would not use any other chemicals due to the dangers other than this. http://members.tripod.com/~h_syriacus/tetraploidy.htm
Brian,
Look up DMSO on the web. It's widely used everywhere but the US. I first heard about it back in the 80's as an analgesic treatment for sprains. It is prescribed in the US for a bladder condition. Keep in mind that all chemicals have their associated dangers: http://www.dhmo.org :)
-Joe G.
Joe the site shows some interesting fact but I had to laugh out loud when I saw the impacts DMSO has on the enviorment it is blaming this one chemical for the 2004 tsunami as well as hurricane Katrina. I have heard of streaching it but man. That would make this one of the most dangerous and powerful chemicals on the planet if it was the real cause of such disasters.
The chemical is not extremely safe and is not overly extremely dangerous. It just needs to be handled with respect.
After reading the website more I believe the person who is running the site has a personal issue with DMSO LOL. Some of the stuff sounds very scientific then it goes off into a crazy direction. As though this chemical is the cause of all bad things on the plant he even ties it into school shootings as well as being used in cult rituals and also used by the KKK and NAACP during rallies and marches LOL. This site is worth looking at for the laughs alone. I really thing the person who made the site has gone overboard a bit with this one.
